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Human GRB10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GRB10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GRB10 antibodies →
$220.00each
Catalog Number: LS-G77500-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GRB10 (NM_001001549) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Grb-10 | GRBIR | GRB-IR | GRB10 adapter protein | GRB10 adaptor protein | IRBP | Maternally expressed gene 1 | MEG1 | KIAA0207 | RSS | GRB10
Origin Species
Human
NCBI GenBank
AC080092
SwissProt
Q13322

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALAGCPDSF LHHPYYQDKV EQTPRSQQDP AGPGLPAQSD RLANHQEDDV DLEALVNDMN
61ASLESLYSAC SMQSDTVPLL QNGQHARSQP RASGPPRSIQ PQVSPRQRVQ RSQPVHILAV
121RRLQEEDQQF RTSSLPAIPN PFPELCGPGS PPVLTPGSLP PSQAAAKQDV KVFSEDGTSK
181VVEILADMTA RDLCQLLVYK SHCVDDNSWT LVEHHPHLGL ERCLEDHELV VQVESTMASE
241SKFLFRKNYA KYEFFKNPMN FFPEQMVTWC QQSNGSQTQL LQEPRHLQLL ADLEDSNIFS
301LIAGRKQYNA PTDHGLCIKP NKVRNETKEL RLLCAEDEQT RTCWMTAFRL LKYGMLLYQN
361YRIPQQRKAL LSPFSTPVRS VSENSLVAMD FSGQTGRVIE NPAEAQSAAL EEGHAWRKRS
421TRMNILGSQS PLHPSTLSTV IHRTQHWFHG RISREESHRI IKQQGLVDGL FLLRDSQSNP
481KAFVLTLCHH QKIKNFQILP CEDDGQTFFS LDDGNTKFSD LIQLVDFYQL NKGVLPCKLK
541HHCIRVAL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

548 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.