LSBio
Human Gravin / AKAP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human Gravin / AKAP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AKAP12 antibodies →
$220.00each
Catalog Number: LS-G76285-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AKAP12 (NM_144497) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A-kinase anchor protein 12 | AKAP-12 | AKAP250 | AKAP 250 | AKAP12 | Gravin | Kinase scaffold protein gravin | Myasthenia gravis autoantigen | SSeCKS
Origin Species
Human
NCBI GenBank
NM_005100
SwissProt
Q02952

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
181.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGTITITVG QRDSEDVSKR DSDKEMATKS AVVHDITDDG QEETPEIIEQ IPSSESNLEE
61LTQPTESQAN DIGFKKVFKF VGFKFTVKKD KTEKPDTVQL LTVKKDEGEG AAGAGDHKDP
121SLGAGEAASK ESEPKQSTEK PEETLKREQS HAEISPPAES GQAVEECKEE GEEKQEKEPS
181KSAESPTSPV TSETGSTFKK FFTQGWAGWR KKTSFRKPKE DEVEASEKKK EQEPEKVDTE
241EDGKAEVASE KLTASEQAHP QEPAESAHEP RLSAEYEKVE LPSEEQVSGS QGPSEEKPAP
301LATEVFDEKI EVHQEEVVAE VHVSTVEERT EEQKTEVEET AGSVPAEELV EMDAEPQEAE
361PAKELVKLKE TCVSGEDPTQ GADLSPDEKV LSKPPEGVVS EVEMLSSQER MKVQGSPLKK
421LFTSTGLKKL SGKKQKGKRG GGDEESGEHT QVPADSPDSQ EEQKGESSAS SPEEPEEITC
481LEKGLAEVQQ DGEAEEGATS DGEKKREGVT PWASFKKMVT PKKRVRRPSE SDKEDELDKV
541KSATLSSTES TASEMQEEMK GSVEEPKPEE PKRKVDTSVS WEALICVGSS KKRARRGSSS
601DEEGGPKAMG GDHQKADEAG KDKETGTDGI LAGSQEHDPG QGSSSPEQAG SPTEGEGVST
661WESFKRLVTP RKKSKSKLEE KSEDSIAGSG VEHSTPDTEP GKEESWVSIK KFIPGRRKKR
721PDGKQEQAPV EDAGPTGANE DDSDVPAVVP LSEYDAVERE KMEAQQAQKS AEQPEQKAAT
781EVSKELSESQ VHMMAAAVAD GTRAATIIEE RSPSWISASV TEPLEQVEAE AALLTEEVLE
841REVIAEEEPP TVTEPLPENR EARGDTVVSE AELTPEAVTA AETAGPLGAE EGTEASAAEE
901TTEMVSAVSQ LTDSPDTTEE ATPVQEVEGG VPDIEEQERR TQEVLQAVAE KVKEESQLPG
961TGGPEDVLQP VQRAEAERPE EQAEASGLKK ETDVVLKVDA QEAKTEPFTQ GKVVGQTTPE
1021SFEKAPQVTE SIESSELVTT CQAETLAGVK SQEMVMEQAI PPDSVETPTD SETDGSTPVA
1081DFDAPGTTQK DEIVEIHEEN EVASGTQSGG TEAEAVPAQK ERPPAPSSFV FQEETKEQSK
1141MEDTLEHTDK EVSVETVSIL SKTEGTQEAD QYADEKTKDV PFFEGLEGSI DTGITVSREK
1201VTEVALKGEG TEEAECKKDD ALELQSHAKS PPSPVEREMV VQVEREKTEA EPTHVNEEKL
1261EHETAVTVSE EVSKQLLQTV NVPIIDGAKE VSSLEGSPPP CLGQEEAVCT KIQVQSSEAS
1321FTLTAAAEEE KVLGETANIL ETGETLEPAG AHLVLEEKSS EKNEDFAAHP GEDAVPTGPD
1381CQAKSTPVIV SATTKKGLSS DLEGEKTTSL KWKSDEVDEQ VACQEVKVSV AIEDLEPENG
1441ILELETKSSK LVQNIIQTAV DQFVRTEETA TEMLTSELQT QAHVIKADSQ DAGQETEKEG
1501EEPQASAQDE TPITSAKEES ESTAVGQAHS DISKDMSEAS EKTMTVEVEG STVNDQQLEE
1561VVLPSEEEGG GAGTKSVPED DGHALLAERI EKSLVEPKED EKGDDVDDPE NQNSALADTD
1621ASGGLTKESP DTNGPKQKEK EDAQEVELQE GKVHSESDKA ITPQAQEELQ KQERESAKSE
1681LTES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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