LSBio
Human GPR150 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GPR150 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GPR150 antibodies →
$220.00each
Catalog Number: LS-G88497-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR 150(GPR150) (NM_199243) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 150 | GPR150 | PGR11 | GPRv40
Origin Species
Human
NCBI GenBank
NM_199243
SwissProt
Q8NGU9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDLFSPSIL PPAPNISVPI LLGWGLNLTL GQGAPASGPP SRRVRLVFLG VILVVAVAGN
61TTVLCRLCGG GGPWAGPKRR KMDFLLVQLA LADLYACGGT ALSQLAWELL GEPRAATGDL
121ACRFLQLLQA SGRGASAHLV VLIALERRRA VRLPHGRPLP ARALAALGWL LALLLALPPA
181FVVRGDSPSP LPPPPPPTSL QPGAPPAARA WPGERRCHGI FAPLPRWHLQ VYAFYEAVAG
241FVAPVTVLGV ACGHLLSVWW RHRPQAPAAA APWSASPGRA PAPSALPRAK VQSLKMSLLL
301ALLFVGCELP YFAARLAAAW SSGPAGDWEG EGLSAALRVV AMANSALNPF VYLFFQAGDC
361RLRRQLRKRL GSLCCAPQGG AEDEEGPRGH QALYRQRWPH PHYHHARREP LDEGGLRPPP
421PRPRPLPCSC ESAF
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

434 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.