GPI Antibody
Catalog Number: LS-C781868-100
100 µg / $587.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GPI Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76158-20
Catalog Number: LS-G76158
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Glucose 6 phosphate isomerase(GPI) (NM_001184722) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AMF | Autocrine motility factor | Glucose phosphate isomerase | Hexose monophosphate isomerase | Hexosephosphate isomerase | GPI | Neuroleukin | Oxoisomerase | Phosphoglucose isomerase | Phosphohexomutase | Phosphohexose isomerase | SA36 | PGI | SA-36 | Glucose-6-phosphate isomerase | GNPI | PHI | Phosphosaccharomutase | Sperm antigen 36 | Sperm antigen-36
- Origin Species
- Human
- NCBI GenBank
- NM_000175
- SwissProt
- P06744
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVALCSLQHL GSSDPRALPT LPTATSGQRP AKRRRKSPAM AALTRDPQFQ KLQQWYREHR61SELNLRRLFD ANKDRFNHFS LTLNTNHGHI LVDYSKNLVT EDVMRMLVDL AKSRGVEAAR121ERMFNGEKIN YTEGRAVLHV ALRNRSNTPI LVDGKDVMPE VNKVLDKMKS FCQGPLMVTE181ALKPYSSGGP RVWYVSNIDG THIAKTLAQL NPESSLFIIA SKTFTTQETI TNAETAKEWF241LQAAKDPSAV AKHFVALSTN TTKVKEFGID PQNMFEFWDW VGGRYSLWSA IGLSIALHVG301FDNFEQLLSG AHWMDQHFRT TPLEKNAPVL LALLGIWYIN CFGCETHAML PYDQYLHRFA361AYFQQGDMES NGKYITKSGT RVDHQTGPIV WGEPGTNGQH AFYQLIHQGT KMIPCDFLIP421VQTQHPIRKG LHHKILLANF LAQTEALMRG KSTEEARKEL QAAGKSPEDL ERLLPHKVFE481GNRPTNSIVF TKLTPFMLGA LVAMYEHKIF VQGIIWDINS FDQWGVELGK QLAKKIEPEL541DGSAQVTSHD ASTNGLINFI KQQREARVQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
569 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
