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Human GOLGA6A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GOLGA6A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G84763-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GOLGA6(GOLGA6A) (NM_001038640) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLP | Golgin linked to PML | Golgin subfamily A member 6A | Golgin-like protein | GOLGA6 | GOLGA6A | Golgin A6 family | member A
Origin Species
Human
SwissProt
Q9NYA3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWPQPYLPPH PMMLEESRQN KLAAAKKKLK EYQQRKSPGI PAGAKTKKKK TDSSPETTTS
61GGCHSPGDSQ YQELAVALES SSVTISQLNE NIESLKQQKK QVEHQLEEAK KTNNEIHKAQ
121MERLETINIL TLEKADLKTT LYHTKRAARH FEEESKDLAG RLQYSLQRIQ ELERALCAVS
181TQQQEEDRSS SCREAVLQRW LQQTIKERAL LNAHVTQVTE SLKQVQLERD EYAKHIKGER
241ARWQERMWKM SVEARTLKEE KKRDIHRIQE LERSLSELKN QMAEPPSLAP PAVTSVVEQL
301QDEAKHLRQE VEGLEGKLQS QVENNQALSL LSKEQKQRLQ EQEEMLREQE AQRVREQERL
361CEQNERLREQ QKTLQEQGER LRKQEQRLRK QEERLRKEEE RLQKQEKRLW DQEERLWKKE
421ERLQKQEERL ALSQNHKLDK QLAEPQCSFE DLNNEKKSAL QLEQQVKELQ EKLDEEHLEA
481ASHQNQQLET QLSLVALPGE GDGGQHLDSE EEEAPRPTPN IPEDLESREA TSSFMDLPKE
541KADGTEQVER RELGFVQPSG VTDGMRESFT VYESQGAVPN TRHQEMEDVI RLAQKEEEMK
601VKLLELQELV LPLVGNHEGH GKFLIAAQNP ADEPTPGAPA PQELGAAGEQ DVFYEVSLDN
661NVEPAPGAAR EGSPHDNPTV QQIVQLSPVM QDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

693 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.