GCLC Antibody
Catalog Number: LS-C746772
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GCLC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76180-20
Catalog Number: LS-G76180
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GCLC (NM_001197115) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCL | GCS heavy chain | GCLC | Gamma-ECS | GCS | GLCL | GLCLC
- Origin Species
- Human
- NCBI GenBank
- NM_001498
- SwissProt
- P48506
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLLSQGSPL SWEETKRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE VEYMLVSFDH61ENKKVRLVLS GEKVLETLQE KGERTNPNHP TLWRPEYGSY MIEGTPGQPY GGTMSEFNTV121EANMRKRRKE ATSILEENQA LCTITSFPST LTRNIRHRRG EKVVINVPIF KDKNTPSPFI181ETFTEDDEAS RASKPDHIYM DAMGFGMGNC CLQVTFQACS ISEARYLYDQ LATICPIVMA241LSAASPFYRG YVSDIDCRWG VISASVDDRT REERGLEPLK NNNYRISKSR YDSIDSYLSK301CGEKYNDIDL TIDKEIYEQL LQEGIDHLLA QHVAHLFIRD PLTLFEEKIH LDDANESDHF361ENIQSTNWQT MRFKPPPPNS DIGWRVEFRP MEVQLTDFEN SAYVVFVVLL TRVILSYKLD421FLIPLSKVDE NMKVAQKRDA VLQGMFYFRK DICKGGNAVV DGCGKAQNST ELAAEEYTLM481SIDTIINGKE GVFPGLIPIL NSYLENMEVD VDTRCSILNY LKLIKKRASG ELMTVARWMR541EFIANHPDYK QDSVITDEMN YSLILKCNQI ANELCECPEL LGSAFRKVKY SGSKTDSSNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
599 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
