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Human GAPVD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GAPVD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GAPVD1 antibodies →
$220.00each
Catalog Number: LS-G83458-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GAPex 5(GAPVD1) (NM_015635) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434C212 | GAPEX5 | KIAA1521 | GAPex-5 | GAPVD1 | RAP6 | Rab5-activating protein 6
Origin Species
Human
NCBI GenBank
AB040954
SwissProt
Q14C86

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
166 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVKLDIHTLA HHLKQERLYV NSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII
61TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL
121NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI
181LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPSQ QEKLFGEKGS
241DRFRQKVQEM VESNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG
301EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGSEEGD
361PRTKSSLGKF DKSCVAAFLD VVIGGRAVET PPLSSVNLLE GLSRTVVYIT YSQLITLVNF
421MKSVMSGDQL REDRMALDNL LANLPPAKPG KSSSLEMTPY NTPQLSPATT PANKKNRLPI
481ATRSRSRTNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL
541SDGGQGDVPV DENKLHGKPD KTLRFSLCSD NLEGISEGPS NRSNSVSSLD LEGESVSELG
601AGPSGSNGVE ALQLLEHEQA TTQDNLDDKL RKFEIRDMMG LTDDRDISET VSETWSTDVL
661GSDFDPNIDE DRLQEIAGAA AENMLGSLLC LPGSGSVLLD PCTGSTISET TSEAWSVEVL
721PSDSEAPDLK QEERLQELES CSGLGSTSDD TDVREVSSRP STPGLSVVSG ISATSEDIPN
781KIEDLRSECS SDFGGKDSVT SPDMDEITHD FLYILQPKQH FQHIEAEADM RIQLSSSAHQ
841LTSPPSQSES LLAMFDPLSS HEGASAVVRP KVHYARPSHP PPDPPILEGA VGGNEARLPN
901FGSHVLTPAE MEAFKQRHSY PERLVRSRSS DIVSSVRRPM SDPSWNRRPG NEERELPPAA
961AIGATSLVAA PHSSSSSPSK DSSRGETEER KDSDDEKSDR NRPWWRKRFV SAMPKAPIPF
1021RKKEKQEKDK DDLGPDRFST LTDDPSPRLS AQAQVAEDIL DKYRNAIKRT SPSDGAMANY
1081ESTEVMGDGE SAHDSPRDEA LQNISADDLP DSASQAAHPQ DSAFSYRDAK KKLRLALCSA
1141DSVAFPVLTH STRNGLPDHT DPEDNEIVCF LKVQIAEAIN LQDKNLMAQL QETMRCVCRF
1201DNRTCRKLLA SIAEDYRKRA PYIAYLTRCR QGLQTTQAHL ERLLQRVLRD KEVANRYFTT
1261VCVRLLLESK EKKIREFIQD FQKLTAADDK TAQVEDFLQF LYGAMAQDVI WQNASEEQLQ
1321DAQLAIERSV MNRIFKLAFY PNQDGDILRD QVLHEHIQRL SKVVTANHRA LQIPEVYLRE
1381APWPSAQSEI RTISAYKTPR DKVQCILRMC STIMNLLSLA NEDSVPGADD FVPVLVFVLI
1441KANPPCLLST VQYISSFYAS CLSGEESYWW MQFTAAVEFI KTIDDRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1487 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.