Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37 degrees C with 0.5ug/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20ug/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)