FREM3 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G130474-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FREM3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96042-20
Catalog Number: LS-G96042
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FREM3 (NM_001168235) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001168235
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 238.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGASRHPTG TPRQLLVALA CLLLSRPALQ GRASSLGTEP DPALYLPARG ALDGTRPDGP61SVLIANPGLR VPLGRSLWLD PLRDLVIGVQ PGDRCEVTVL DALPRLKGAL SPRRFPCTFG121PRQVQYTHFG SHSPGRARVL LQLRYDAPTH TLVLPFTLAV DLVFSQLELV TRNRPLVVEK181LRSWSRAIDR RVLDFASLKS GATATRRCRL TPLPHEDGPL PKYGRLVDAV GAPLPRGKGV241DCEAFLRAGV RYQHTATSSP NRDYVPMMVE LLGPEGQDAG SAGVLVREHF QLLVRIRGGA301ENTPPRPSFM ATMMMEVDPL VLTALTPDAL AAEDVESDPG DLVFNILNAP THPPGHPGQQ361GYVVSTDDPL GLPVSFFTQQ ELRELKIAYQ PPAENSHGER LFQLELEVVD GDGAASDPFA421FMVTVKSMNT LVPVASHNRG LVLFEGQSRP LSSTHSIPIS DKDNLEEVKM AAVRGLRHGQ481LVVFGAPAGC KYFTPADLAA GRVVYQHDGS NTYSDNIIFR MEDGHHQVDF LFPLTILPVD541DEPPMVNTNT GLSLTEGQVV QISPFVLSAT DIDSEDSTIH FVLENQPLKG NEEEPQWELA601PGSSHSGHYL GDLLLQQAEL PLSTEDEDWH YMEKEGLYEK VVTEWLQRDI MEGRLFYRHL661GPHSPQSVMV QLAFHVQDDH DPPNLSKQHI FTIKVQPVDI LSPQLYPGTT LEMTVQEYQL721THFQKNFLRY IDQDSDDQNL WYTLLTLPTD TDGNHQVRAG EIVLTDSPDT LIMHFTQAQV781NQHKVAYQPP QKLGIAPRVV QFTYQVEDAA GNSVPGTFTL FLQPVDNQPP EVTNRGFAIL841EGGSFNLSSN ELHVTDPDTD IDQIVFILVR GPQHGHLQYF KRCMVPGESF MQADVINGSV901SYQHGRDQTT TSDTFHLEVS DGVHHIPITI PISVHPNVAN RSPRISLRSS SLLDVSIDVL961ENKATEITMG VIHGKRKDVG DLMLSFIVKD SPKLGTILVN GLPTERFTQE DLINGRVAYA1021HTAGEVGFQK QHDAFSLILS KDSYQWVVGN SIIEKVQVQV TVLPVDNVGP KVFVGESFIV1081YEGEKNSLTL QHLHVEDVDT HQDELLCTVT SQPASGYLEK IASAPGSKMS QSGSPISAFS1141LRDIQVRHIN YVQSIHKGVE PQEDQFTFYC SDGINFSPNV FFPIIILPTN DEQPKLFAHE1201FKVLEGMSLV IDTQLLNGAD ADLPPNELHF QLTALPRHGR IIQQLATGSQ PIHSFTLKEI1261QEASTIVYEH DDSETKEDSF EVWLSDGKHT THRKVPIVVT LVDDETPHLT VNNGLKVEKG1321HSEIITNRIL KATDLDSDDK SLSFVLHSGP QQGLLQRLRK PRGEVRNNLT LGMNFTQDEI1381NRGLICYIHT GQEGIVDIIK FDVTDGVNTL TDHYFYVTIG NLDSVFPEVI SKRITLIEGA1441RVTLTNNLLT NSDINSSDEH HFSITRAPSL GHLESSDYAG EPIASFTQLQ LASNKISYVH1501TSNDEKKMDS FEFQVIGELY PVFRTFRIFI TDVDNKKPIL TIHRLTLQKE DSQLITLLEL1561TVEDSDTPDD LILFTITQVP MHGKILYNGS RPVTTFTKQD LNKNLISYKH DGSETTEDSF1621SLTVTDGTHT DFYVLPDTAL ATHKPQVMRV QIRSLDNRLP QITTNRGAPA LKRLHTGHMG1681FLITSKSLKA EDQDSPHRLL KYKVTRGPEH GFIIKTGLGN QSTRVFTQAD IDEMKISYVL1741NEGSNASKDI FYFSVEDNGG NKLTNQPFHL NWAWICLEKE YYIVDEDSTF LEVTLTRRGY1801LGETSFISIG TKDETAKKDK DFKWKTNKQI QFNPGQTTAT WRVRIIPDNE YETSETFQII1861LSEPLMAVLE FPEMATVEIV DPGDESTVYI PEAEYKIEED IGELLIPVRR SGDASQELIV1921ICSTRQGSAT GTISSTVLFS DYISRPEDHT SILHFDKNET QKTCQVLIID DSLYEEEESF1981SVSLRLPVGG QLGARFPTTK VTILADRYDE PVLHFGDAEY HVNESARYVE VCVWRRGTDL2041SQPSSIAVRS RKSEQESAEA GTDYVGISRN LDFAPGVRMQ TFQVTILDDL GQPTLEGPEK2101FELLLQMPMG AVLGEPNKTT IFIEDTITDC KQSACSSFDSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
2139 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
