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Human FLII / FLI Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FLII / FLI Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FLII antibodies →
$153.00each
Catalog Number: LS-G81400-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Flightless I(FLII) (NM_002018) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLI | FLIL | Protein flightless-1 homolog | FLII
Origin Species
Human
NCBI GenBank
NM_002018
SwissProt
Q13045

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
144.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE ELAALQKLEH
61LSVSHNNLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI FKLDDLSVLD LSHNQLTECP
121RELENAKNML VLNLSHNSID TIPNQLFINL TDLLYLDLSE NRLESLPPQM RRLVHLQTLV
181LNGNPLLHAQ LRQLPAMTAL QTLHLRSTQR TQSNLPTSLE GLSNLADVDL SCNDLTRVPE
241CLYTLPSLRR LNLSSNQITE LSLCIDQWVH VETLNLSRNQ LTSLPSAICK LSKLKKLYLN
301SNKLDFDGLP SGIGKLTNLE EFMAANNNLE LVPESLCRCP KLRKLVLNKN HLVTLPEAIH
361FLTEIEVLDV RENPNLVMPP KPADRAAEWY NIDFSLQNQL RLAGASPATV AAAAAAGSGP
421KDPMARKMRL RRRKDSAQDD QAKQVLKGMS DVAQEKNKKQ EESADARAPS GKVRRWDQGL
481EKPRLDYSEF FTEDVGQLPG LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS
541LNWEIYYWIG GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY
601IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGT SLDPRFVFLL DRGLDIYVWR
661GAQATLSSTT KARLFAEKIN KNERKGKAEI TLLVQGQELP EFWEALGGEP SEIKKHVPED
721FWPPQPKLYK VGLGLGYLEL PQINYKLSVE HKQRPKVELM PRMRLLQSLL DTRCVYILDC
781WSDVFIWLGR KSPRLVRAAA LKLGQELCGM LHRPRHATVS RSLEGTEAQV FKAKFKNWDD
841VLTVDYTRNA EAVLQSPGLS GKVKRDAEKK DQMKADLTAL FLPRQPPMSL AEAEQLMEEW
901NEDLDGMEGF VLEGKKFARL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE KKEDKEEKAE
961GKEGEEATAE AEEKQPEEDF QCIVYFWQGR EASNMGWLTF TFSLQKKFES LFPGKLEVVR
1021MTQQQENPKF LSHFKRKFII HRGKRKAVQG AQQPSLYQIR TNGSALCTRC IQINTDSSLL
1081NSEFCFILKV PFESEDNQGI VYAWVGRASD PDEAKLAEDI LNTMFDTSYS KQVINEGEEP
1141ENFFWVGIGA QKPYDDDAEY MKHTRLFRCS NEKGYFAVTE KCSDFCQDDL ADDDIMLLDN
1201GQEVYMWVGT QTSQVEIKLS LKACQVYIQH MRSKEHERPR RLRLVRKGNE QHAFTRCFHA
1261WSAFCKALA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1269 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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