LSBio
Human FECH / Ferrochelatase Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FECH / Ferrochelatase Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FECH antibodies →
$220.00each
Catalog Number: LS-G75906-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FECH (NM_001012515) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FCE | FECH | Heme synthase | EPP | Heme synthetase | Protoheme ferro-lyase | Protoporphyria | Ferrochelatase | mitochondrial
Origin Species
Human
NCBI GenBank
NM_000140
SwissProt
P22830

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRSLGANMAA ALRAAGVLLR DPLASSSWRV CQPWRWKSGA AAAAVTTETA QHAQGAKPQV
61QPQKRYESNI RKPKTGILML NMGGPETLGD VHDFLLRLFL DRDLMTLPIQ NKLAPFIAKR
121RTPKIQEQYR RIGGGSPIKI WTSKQGEGMV KLLDELSPNT APHKYYIGFR YVHPLTEEAI
181EEMERDGLER AIAFTQYPQY SCSTTGSSLN AIYRYYNQVG RKPTMKWSTI DRWPTHHLLI
241QCFADHILKE LDHFPLEKRS EVVILFSAHS LPMSVVNRGD PYPQEVSATV QKVMERLEYC
301NPYRLVWQSK VGPMPWLGPQ TDESIKGLCE RGRKNILLVP IAFTSDHIET LYELDIEYSQ
361VLAKECGVEN IRRAESLNGN PLFSKALADL VHSHIQSNEL CSKQLTLSCP LCVNPVCRET
421KSFFTSQQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

429 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.