FBXW12 Antibody (HRP)
Catalog Number: LS-C399401
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FBXW12 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G94709-20
Catalog Number: LS-G94709
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FBXW12 (NM_207102) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FBXO35 | FBXW12 | F-box only protein 35 | FBW12 | FBXO12
- Origin Species
- Human
- SwissProt
- Q6X9E4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEIRLPDLAL KRIFSFLDLF GLLQVSQVNK HWNRIADSDY LWRSLSLQRW DCSNFTNQHL61GTHTWKQFFL HQRRKELRLA LAQPHNFIYK VTKNIAFETE LAYLSGNRLT VDEQEKSIIC121SVSPKQELCA WDVQEGTMIW SSPVQEFHFS NLVTLPQMHL AITMDRKKTI KVWNCQDRDA181LAVLPMPQPC YCMEAYLTKD GPFLMVGDAA GDIYTFTLPG LRDVSKVTAF QYGIVLLHCS241PDKKWVFACG TYSRTLPQVF LTESLLRPSE GSDPLSTFLP HKLCASACWT PKVKNRITLM301SQSSTGKKTE FITFDLPTKK TGGQTVIQAY EIASFQVAAH LKCPIWMGAS DGYMIVFTSG361PYLLLFSITG FLLQRFEDHQ AAINNFWVDP CYVLTTSENS VHVYMWEEGG RHPYLRSCCH421LENTWHDHTT DSCISSVMCD NASIVLRVRK VSDSSILVMY SLNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
464 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
