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Human FAM73B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FAM73B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FAM73B antibodies →
$153.00each
Catalog Number: LS-G87075-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAM73B(FAM73B) (NM_032809) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf54 | FAM73B | Protein FAM73B
Origin Species
Human
NCBI GenBank
NM_032809
SwissProt
Q7L4E1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFRRAEGTS MIQALAMTVA EIPVFLYTTF GQSAFSQLRL TPGLRKVLFA TALGTVALAL
61AAHQLKRRRR RKKQVGPEMG GEQLGTVPLP ILLARKVPSV KKGYSSRRVQ SPSSKSNDTL
121SGISSIEPSK HSGSSHSVAS MMAVNSSSPT AACSGLWDAR GMEESLTTSD GNAESLYMQG
181MELFEEALQK WEQALSVGQR GDSGSTPMPR DGLRNPETAS EPLSEPESQR KEFAEKLESL
241LHRAYHLQEE FGSTFPADSM LLDLERTLML PLTEGSLRLR ADDEDSLTSE DSFFSATELF
301ESLQTGDYPI PLSRPAAAYE EALQLVKEGR VPCRTLRTEL LGCYSDQDFL AKLHCVRQAF
361EGLLEDKSNQ LFFGKVGRQM VTGLMTKAEK SPKGFLESYE EMLSYALRPE TWATTRLELE
421GRGVVCMSFF DIVLDFILMD AFEDLENPPA SVLAVLRNRW LSDSFKETAL ATACWSVLKA
481KRRLLMVPDG FISHFYSVSE HVSPVLAFGF LGPKPQLAEV CAFFKHQIVQ YLRDMFDLDN
541VRYTSLPALA DDILQLSRRR SEILLGYLGV PAASSAGVNG ALPRENGPLG ELQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

593 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.