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Human FAM65C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FAM65C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FAM65C antibodies →
$220.00each
Catalog Number: LS-G95448-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAM65C (NM_080829) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf175 | C20orf176 | DJ530I15.2 | Protein FAM65C | DJ530I15.3 | FAM65C
Origin Species
Human
NCBI GenBank
NM_080829
SwissProt
Q96MK2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVRLRFLSP GDTGAVGVVG RSASFAGFSS AQSRRIAKSI NRNSVRSRMP AKSSKMYGTL
61RKGSVCADPK PQQVKKIFEA LKRGLKEYLC VQQAELDHLS GRHKDTRRNS RLAFYYDLDK
121QTRCVERHIR KMEFHISKVD ELYEDYCIQC RLRDGASSMQ RAFARCPPSR AARESLQELG
181RSLHECAEDM WLIEGALEVH LGEFHIRMKG LVGYARLCPG DHYEVLMRLG RQRWKLKGRI
241ESDDSQTWDE EEKAFIPTLH ENLDIKVTEL RGLGSLAVGA VTCDIADFFT TRPQVIVVDI
301TELGTIKLQL EVQWNPFDTE SFLVSPSPTG KFSMGSRKGS LYNWTPPSTP SFRERYYLSV
361LQQPTQQALL LGGPRATSIL SYLSDSDLRG PSLRSQSQEL PEMDSFSSED PRDTETSTSA
421STSDVGFLPL TFGPHASIEE EAREDPLPPG LLPEMAHLSG GPFAEQPGWR NLGGESPSLP
481QGSLFHSGTA SSSQNGHEEG ATGDREDGPG VALEGPLQEV LELLRPTDST QPQLRELEYQ
541VLGFRDRLKP CRARQEHTSA ESLMECILES FAFLNADFAL DELSLFGGSQ GLRKDRPLPP
601PSSLKASSRE LTAGAPELDV LLMVHLQVCK ALLQKLASPN LSRLVQECLL EEVAQQKHVL
661ETLSVLDFEK VGKATSIEEI IPQASRTKGC LKLWRGCTGP GRVLSCPATT LLNQLKKTFQ
721HRVRGKYPGQ LEIACRRLLE QVVSCGGLLP GAGLPEEQII TWFQFHSYLQ RQSVSDLEKH
781FTQLTKEVTL IEELHCAGQA KVVRKLQGKR LGQLQPLPQT LRAWALLQLD GTPRVCRAAS
841ARLAGAVRNR SFREKALLFY TNALAENDAR LQQAACLALK HLKGIESIDQ TASLCQSDLE
901AVRAAARETT LSFGEKGRLA FEKMDKLCSE QREVFCQEAD VEITIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

946 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.