LSBio
Human FAK / Focal Adhesion Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FAK / Focal Adhesion Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PTK2 antibodies →
$220.00each
Catalog Number: LS-G75952-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAK(PTK2) (NM_005607) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK | PTK2
Origin Species
Human
NCBI GenBank
NM_005607
SwissProt
Q05397

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MISADCNLCL PEYDRYLASS KIMAAAYLDP NLNHTPNSST KTHLGTGMER SPGAMERVLK
61VFHYFESNSE PTTWASIIRH GDATDVRGII QKIVDSHKVK HVACYGFRLS HLRSEEVHWL
121HVDMGVSSVR EKYELAHPPE EWKYELRIRY LPKGFLNQFT EDKPTLNFFY QQVKSDYMLE
181IADQVDQEIA LKLGCLEIRR SYWEMRGNAL EKKSNYEVLE KDVGLKRFFP KSLLDSVKAK
241TLRKLIQQTF RQFANLNREE SILKFFEILS PVYRFDKECF KCALGSSWII SVELAIGPEE
301GISYLTDKGC NPTHLADFTQ VQTIQYSNSE DKDRKGMLQL KIAGAPEPLT VTAPSLTIAE
361NMADLIDGYC RLVNGTSQSF IIRPQKEGER ALPSIPKLAN SEKQGMRTHA VSVSETDDYA
421EIIDEEDTYT MPSTRDYEIQ RERIELGRCI GEGQFGDVHQ GIYMSPENPA LAVAIKTCKN
481CTSDSVREKF LQEALTMRQF DHPHIVKLIG VITENPVWII MELCTLGELR SFLQVRKYSL
541DLASLILYAY QLSTALAYLE SKRFVHRDIA ARNVLVSSND CVKLGDFGLS RYMEDSTYYK
601ASKGKLPIKW MAPESINFRR FTSASDVWMF GVCMWEILMH GVKPFQGVKN NDVIGRIENG
661ERLPMPPNCP PTLYSLMTKC WAYDPSRRPR FTELKAQLST ILEEEKAQQE ERMRMESRRQ
721ATVSWDSGGS DEAPPKPSRP GYPSPRSSEG FYPSPQHMVQ TNHYQVSGYP GSHGITAMAG
781SIYPGQASLL DQTDSWNHRP QEIAMWQPNV EDSTVLDLRG IGQVLPTHLM EERLIRQQQE
841MEEDQRWLEK EERFLKPDVR LSRGSIDRED GSLQGPIGNQ HIYQPVGKPD PAAPPKKPPR
901PGAPGHLGSL ASLSSPADSY NEGVKLQPQE ISPPPTANLD RSNDKVYENV TGLVKAVIEM
961SSKIQPAPPE EYVPMVKEVG LALRTLLATV DETIPLLPAS THREIEMAQK LLNSDLGELI
1021NKMKLAQQYV MTSLQQEYKK QMLTAAHALA VDAKNLLDVI DQARLKMLGQ TRPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1074 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.