FAK / Focal Adhesion Kinase Antibody
Catalog Number: LS-C285815
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Chicken
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FAK / Focal Adhesion Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G75952-20
Catalog Number: LS-G75952
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAK(PTK2) (NM_005607) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK | PTK2
- Origin Species
- Human
- NCBI GenBank
- NM_005607
- SwissProt
- Q05397
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MISADCNLCL PEYDRYLASS KIMAAAYLDP NLNHTPNSST KTHLGTGMER SPGAMERVLK61VFHYFESNSE PTTWASIIRH GDATDVRGII QKIVDSHKVK HVACYGFRLS HLRSEEVHWL121HVDMGVSSVR EKYELAHPPE EWKYELRIRY LPKGFLNQFT EDKPTLNFFY QQVKSDYMLE181IADQVDQEIA LKLGCLEIRR SYWEMRGNAL EKKSNYEVLE KDVGLKRFFP KSLLDSVKAK241TLRKLIQQTF RQFANLNREE SILKFFEILS PVYRFDKECF KCALGSSWII SVELAIGPEE301GISYLTDKGC NPTHLADFTQ VQTIQYSNSE DKDRKGMLQL KIAGAPEPLT VTAPSLTIAE361NMADLIDGYC RLVNGTSQSF IIRPQKEGER ALPSIPKLAN SEKQGMRTHA VSVSETDDYA421EIIDEEDTYT MPSTRDYEIQ RERIELGRCI GEGQFGDVHQ GIYMSPENPA LAVAIKTCKN481CTSDSVREKF LQEALTMRQF DHPHIVKLIG VITENPVWII MELCTLGELR SFLQVRKYSL541DLASLILYAY QLSTALAYLE SKRFVHRDIA ARNVLVSSND CVKLGDFGLS RYMEDSTYYK601ASKGKLPIKW MAPESINFRR FTSASDVWMF GVCMWEILMH GVKPFQGVKN NDVIGRIENG661ERLPMPPNCP PTLYSLMTKC WAYDPSRRPR FTELKAQLST ILEEEKAQQE ERMRMESRRQ721ATVSWDSGGS DEAPPKPSRP GYPSPRSSEG FYPSPQHMVQ TNHYQVSGYP GSHGITAMAG781SIYPGQASLL DQTDSWNHRP QEIAMWQPNV EDSTVLDLRG IGQVLPTHLM EERLIRQQQE841MEEDQRWLEK EERFLKPDVR LSRGSIDRED GSLQGPIGNQ HIYQPVGKPD PAAPPKKPPR901PGAPGHLGSL ASLSSPADSY NEGVKLQPQE ISPPPTANLD RSNDKVYENV TGLVKAVIEM961SSKIQPAPPE EYVPMVKEVG LALRTLLATV DETIPLLPAS THREIEMAQK LLNSDLGELI1021NKMKLAQQYV MTSLQQEYKK QMLTAAHALA VDAKNLLDVI DQARLKMLGQ TRPHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1074 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
