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Human EVI5L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human EVI5L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all EVI5L antibodies →
$153.00each
Catalog Number: LS-G88403-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EVI5L (NM_145245) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EVI5-like protein | EVI5L
Origin Species
Human
NCBI GenBank
BC014111
SwissProt
Q96CN4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASPTLSPDS SSQEALSAPT CSPTSDSENL SPDELELLAK LEEQNRLLEA DSKSMRSMNG
61SRRNSGSSLV SSSSASSNLS HLEEDTWILW GRIANEWEEW RRRKEKLLKE LIRKGIPHHF
121RAIVWQLLCS ATDMPVKNQY SELLKMSSPC EKLIRRDIAR TYPEHEFFKG QDSLGQEVLF
181NVMKAYSLVD REVGYCQGSA FIVGLLLMQM PEEEAFCVFV RLMQEYRLRE LFKPSMAELG
241LCIYQFEYML QEQLPDLNTH FRSQSFHTSM YASSWFLTLF LTTFPLPVAT RVFDIFMYEG
301LEIVFRVGLA LLQVNQAELM QLDMEGMSQY FQRVIPHQFD SCPDKLVLKA YQVKYNPKKM
361KRLEKEYAAM KSKEMEEQIE IKRLRTENRL LKQRIETLEK GQVTRAQEAE ENYVIKRELA
421VVRQQCSSAA EDLQKAQSTI RQLQEQQENP RLTEDFVSHL ETELEQSRLR ETETLGALRE
481MQDKVLDMEK RNSSLPDENN VAQLQEELKA LKVREGQAVA STRELKLQLQ ELSDTWQAHL
541ARGGRWKESP RKLVVGELQD ELMSVRLREA QALAEGRELR QRVVELETQD HIHRNLLNRV
601EAERAALQEK LQYLAAQNKG LQTQLSESRR KQAEAECKSK EEVMAVRLRE ADSMAAVAEM
661RQRIAELEIQ REEGRIQGQL NHSDSSQYIR ELKDQIEELK AEVRLLKGPP PFEDPLAFDG
721LSLARHLDED SLPSSDEELL GVGVGAALQD ALYPLSPRDA RFFRRLERPA KDSEGSSDSD
781ADELAAPYSQ GLDN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

794 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.