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Human EPHA10 / EPH Receptor A10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human EPHA10 / EPH Receptor A10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all EPHA10 antibodies →
$220.00each
Catalog Number: LS-G92399-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EPHA10 (NM_001099439) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EPHA10 | Ephrin type-A receptor 10 | EPH receptor A10 | EphA10s protein
Origin Species
Human
NCBI GenBank
AX166562
SwissProt
Q5JZY3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1METCAGPHPL RLFLCRMQLC LALLLGPWRP GTAEEVILLD SKASQAELGW TALPSNGWEE
61ISGVDEHDRP IRTYQVCNVL EPNQDNWLQT GWISRGRGQR IFVELQFTLR DCSSIPGAAG
121TCKETFNVYY LETEADLGRG RPRLGGSRPR KIDTIAADES FTQGDLGERK MKLNTEVREI
181GPLSRRGFHL AFQDVGACVA LVSVRVYYKQ CRATVRGLAT FPATAAESAF STLVEVAGTC
241VAHSEGEPGS PPRMHCGADG EWLVPVGRCS CSAGFQERGD FCEACPPGFY KVSPRRPLCS
301PCPEHSRALE NASTFCVCQD SYARSPTDPP SASCTRPPSA PRDLQYSLSR SPLVLRLRWL
361PPADSGGRSD VTYSLLCLRC GREGPAGACE PCGPRVAFLP RQAGLRERAA TLLHLRPGAR
421YTVRVAALNG VSGPAAAAGT TYAQVTVSTG PGAPWEEDEI RRDRVEPQSV SLSWREPIPA
481GAPGANDTEY EIRYYEKGQS EQTYSMVKTG APTVTVTNLK PATRYVFQIR AASPGPSWEA
541QSFNPSIEVQ TLGEAASGSR DQSPAIVVTV VTISALLVLG SVMSVLAIWR RPCSYGKGGG
601DAHDEEELYF HFKVPTRRTF LDPQSCGDLL QAVHLFAKEL DAKSVTLERS LGGGRFGELC
661CGCLQLPGRQ ELLVAVHMLR DSASDSQRLG FLAEALTLGQ FDHSHIVRLE GVVTRGSTLM
721IVTEYMSHGA LDGFLRRHEG QLVAGQLMGL LPGLASAMKY LSEMGYVHRG LAARHVLVSS
781DLVCKISGFG RGPRDRSEAV YTTMSGRSPA LWAAPETLQF GHFSSASDVW SFGIIMWEVM
841AFGERPYWDM SGQDVIKAVE DGFRLPPPRN CPNLLHRLML DCWQKDPGER PRFSQIHSIL
901SKMVQDPEPP KCALTTCPRP PTPLADRAFS TFPSFGSVGA WLEALDLCRY KDSFAAAGYG
961SLEAVAEMTA QDLVSLGISL AEHREALLSG ISALQARVLQ LQGQGVQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1008 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.