LSBio
Human EGR4 / EGR-4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human EGR4 / EGR-4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all EGR4 antibodies →
$153.00each
Catalog Number: LS-G75678-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EGR4 (NM_001965) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AT133 | Early growth response 4 | EGR4 | NGFIC | NGFI-C | EGR-4 | PAT133
Origin Species
Human
NCBI GenBank
NM_001965
SwissProt
Q05215

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR LPDSFPRGEC
61PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR RRAMLHLSEF SEPDALLVKS
121TEGCCAEPSA ELPRLPARDA PAATGYPGAG DFLSWALNSC GASGDLADSC FLEGPAPTPP
181PGLSYSGSFF IQAVPEHPHD PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL
241LPGPPDLYSP DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA
301VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS CPAELPAVPA
361NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS GDGGEFLAST QPQLSPLGLR
421SAAAADFPKP LVADIPGSSG VAAPPVPPPP PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA
481KAFACPVESC VRSFARSDEL NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP
541FACDVCGRRF ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

589 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.