ECM2 Antibody (N-Terminus, FITC)
Catalog Number: LS-C473220-100
100 µL (0.5 mg/mL) / $479.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Non-Human Primate
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ECM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89117-20
Catalog Number: LS-G89117
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ECM2 (NM_001393) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ECM2 | Matrix glycoprotein SC1/ECM2 | Extracellular matrix protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_001393
- SwissProt
- O94769
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.79 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIAVLFCFF LLIIFQTDFG KNEEIPRKQR RKIYHRRLRK SSTSHKHRSN RQLGIQQTTV61FTPVARLPIV NFDYSMEEKF ESFSSFPGVE SSYNVLPGKK GHCLVKGITM YNKAVWSPEP121CTTCLCSDGR VLCDETMCHP QRCPQTVIPE GECCPVCSAT VSYSLLSGIA LNDRNEFSGD181SSEQREPTNL LHKQLPPPQV GMDRIVRKEA LQSEEDEEVK EEDTEQKRET PESRNQGQLY241SEGDSRGGDR KQRPGEERRL AHQQQRQGRE EEEDEEEEGE EGEEDEEDEE DPVRGDMFRM301PSRSPLPAPP RGTLRLPSGC SLSYRTISCI NAMLTQIPPL TAPQITSLEL TGNSIASIPD361EAFNGLPNLE RLDLSKNNIT SSGIGPKAFK LLKKLMRLNM DGNNLIQIPS QLPSTLEELK421VNENNLQAID EESLSDLNQL VTLELEGNNL SEANVNPLAF KPLKSLAYLR LGKNKFRIIP481QGLPGSIEEL YLENNQIEEI TEICFNHTRK INVIVLRYNK IEENRIAPLA WINQENLESI541DLSYNKLYHV PSYLPKSLLH LVLLGNQIER IPGYVFGHME PGLEYLYLSF NKLADDGMDR601VSFYGAYHSL RELFLDHNDL KSIPPGIQEM KALHFLRLNN NKIRNILPEE ICNAEEDDDS661NLEHLHLENN YIKIREIPSY TFSCIRSYSS IVLKPQNIKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
699 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
