LSBio
Human DRC7 / CCDC135 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DRC7 / CCDC135 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DRC7 antibodies →
$153.00each
Catalog Number: LS-G92142-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DRC7 (NM_032269) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC135 | FAP50 | C16orf50
Origin Species
Human
NCBI GenBank
NM_032269
SwissProt
Q8IY82

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVLREKVEE EEEAEREEAA EWAEWARMEK MMRPVEVRKE EITLKQETLR DLEKKLSEIQ
61ITVSAELPAF TKDTIDISKL PISYKTNTPK EEHLLQVADN FSRQYSHLCP DRVPLFLHPL
121NECEVPKFVS TTLRPTLMPY PELYNWDSCA QFVSDFLTMV PLPDPLKPPS HLYSSTTVLK
181YQKGNSFDFS TLLCSMLIGS GYDAYCVNGY GSLDLCHMDL TREVCPLTVK PKETIKKEEK
241VLPKKYTIKP PRDLCSRFEQ EQEVKKQQEI RAQEKKRLRE EEERLMEAEK AKPDALHGLR
301VHSWVLVLSG KREVPENFFI DPFTGHSYST QDEHFLGIES LWNHKNYWIN MQDCWNCCKD
361LIFDLGDPVR WEYMLLGTDK SQLSLTEEDD SGINDEDDVE NLGKEDEDKS FDMPHSWVEQ
421IEISPEAFET RCLNGKKVIQ YKRAKLEKWA PYLNSNGLVS RLTTYEDLQC TNILEIKEWY
481QNREDMLELK HINKTTDLKT DYFKPGHPQA LRVHSYKSMQ HEMDRVIEFY ETARVDGLMK
541REETPRTMTE YYQGRPDFLS YRHASFGPRV KKLTLSSAES NPRPIVKITE RFFRNPAKPA
601EEDVAERVFL VAEERIQLRY HCREDHITAS KREFLRRTEV DSKGNKIIMT PDMCISFEVE
661PMEHTKKLLY QYEAMMHLKR EEKLSRHQVW ESELEVLEIL KLREEEEAAH TLTISIYDTK
721RNEKSKEYRE AMERMMHEEH LRQVETQLDY LAPFLAQLPP GEKLTCWQAV RLKDECLSDF
781KQRLINKANL IQARFEKETQ ELQKKQQWYQ ENQVTLTPED EDLYLSYCSQ AMFRIRILEQ
841RLNRHKELAP LKYLALEEKL YKDPRLGELQ KIFA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

874 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.