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Human DOCK11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DOCK11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DOCK11 antibodies →
$220.00each
Catalog Number: LS-G96598-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DOCK11 (NM_144658) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
237.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEVRKFTKR LSKPGTAAEL RQSVSEAVRG SVVLEKAKVV EPLDYENVIA QRKTQIYSDP
61LRDLLMFPME DISISVIGRQ RRTVQSTVPE DAEKRAQSLF VKECIKTYST DWHVVNYKYE
121DFSGDFRMLP CKSLRPEKIP NHVFEIDEDC EKDEDSSSLC SQKGGVIKQG WLHKANVNST
181ITVTMKVFKR RYFYLTQLPD GSYILNSYKD EKNSKESKGC IYLDACIDVV QCPKMRRHAF
241ELKMLDKYSH YLAAETEQEM EEWLITLKKI IQINTDSLVQ EKKETVETAQ DDETSSQGKA
301ENIMASLERS MHPELMKYGR ETEQLNKLSR GDGRQNLFSF DSEVQRLDFS GIEPDIKPFE
361EKCNKRFLVN CHDLTFNILG QIGDNAKGPP TNVEPFFINL ALFDVKNNCK ISADFHVDLN
421PPSVREMLWG SSTQLASDGS PKGSSPESYI HGIAESQLLY IQQGIFSVTN PHPEIFLVAR
481IEKVLQGNIT HCAEPYIKNS DPVKTAQKVH RTAKQVCSRL GQYRMPFAWA ARPIFKDTQG
541SLDLDGRFSP LYKQDSSKLS SEDILKLLSE YKKPEKTKLQ IIPGQLNITV ECVPVDLSNC
601ITSSYVPLKP FEKNCQNITV EVEEFVPEMT KYCYPFTIYK NHLYVYPLQL KYDSQKTFAK
661ARNIAVCVEF RDSDESDASA LKCIYGKPAG SVFTTNAYAV VSHHNQNPEF YDEIKIELPI
721HLHQKHHLLF TFYHVSCEIN TKGTTKKQDT VETPVGFAWV PLLKDGRIIT FEQQLPVSAN
781LPPGYLNLND AESRRQCNVD IKWVDGAKPL LKIKSHLEST IYTQDLHVHK FFHHCQLIQS
841GSKEVPGELI KYLKCLHAME IQVMIQFLPV ILMQLFRVLT NMTHEDDVPI NCTMVLLHIV
901SKCHEEGLDS YLRSFIKYSF RPEKPSAPQA QLIHETLATT MIAILKQSAD FLSINKLLKY
961SWFFFEIIAK SMATYLLEEN KIKLPRGQRF PETYHHVLHS LLLAIIPHVT IRYAEIPDES
1021RNVNYSLASF LKRCLTLMDR GFIFNLINDY ISGFSPKDPK VLAEYKFEFL QTICNHEHYI
1081PLNLPMAFAK PKLQRVQDSN LEYSLSDEYC KHHFLVGLLL XETSIALQDN YEIRYTAISV
1141IKNLLIKHAF DTRYQHKNQQ AKIAQLYLPF VGLLLENIQR LAGRDTLYSC AAMPNSASRD
1201EFPCGFTSPA NRGSLSTDKD TAYGSFQNGH GIKREDSRGS LIPEGATGFP DQGNTGENTR
1261QSSTRSSVSQ YNRLDQYEIR SLLMCYLYIV KMISEDTLLT YWNKVSPQEL INILILLEVC
1321LFHFRYMGKR NIARVHDAWL SKHFGIDRKS QTMPALRNRS GVMQARLQHL SSLESSFTLN
1381HSSTTTEADI FHQALLEGNT ATEVSLTVLD TISFFTQCFK TQLLNNDGHN PLMKKVFDIH
1441LAFLKNGQSE VSLKHVFASL RAFISKFPSA FFKGRVNMCA AFCYEVLKCC TSKISSTRNE
1501ASALLYLLMR NNFEYTKRKT FLRTHLQIII AVSQLIADVA LSGGSRFQES LFIINNFANS
1561DRPMKATAFP AEVKDLTKRI RTVLMATAQM KEHEKDPEML IDLQYSLAKS YASTPELRKT
1621WLDSMAKIHV KNGDFSEAAM CYVHVAALVA EFLHRKKLFP NGCSAFKKIT PNIDEEGAMK
1681EDAGMMDVHY SEEVLLELLE QCVDGLWKAE RYEIISEISK LIVPIYEKRR EFEKLTQVYR
1741TLHGAYTKIL EVMHTKKRLL GTFFRVAFYG QSFFEEEDGK EYIYKEPKLT GLSEISLRLV
1801KLYGEKFGTE NVKIIQDSDK VNAKELDPKY AHIQVTYVKP YFDDKELTER KTEFERNHNI
1861SRFVFEAPYT LSGKKQGCIE EQCKRRTILT TSNSFPYVKK RIPINCEQQI NLKPIDVATD
1921EIKDKTAELQ KLCSSTDVDM IQLQLKLXGC VSVQVNAGPL AYARAFLNDS QASKYPPKKV
1981SELKDMFRKF IQACSIALEL NERLIKEDQV EYHEGLKSNF RDMVKELSDI IHEQILQEDT
2041MHSPWMSNTL HVFCAISGTS SDRGYGSPRY AEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2073 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.