DOC2B Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G117211-100
100 µg (1 mg/mL) / $504.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DOC2B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G85508-20
Catalog Number: LS-G85508
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DOC2B (NM_003585) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DOC2B | DOC2BL | Double C2-like domains | beta | Doc2-beta
- Origin Species
- Hu
- NCBI GenBank
- NM_003585
Protein
- Expression System
- HEK293T
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 45.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPDAGP RAAAPPDAPA61RPAVAGAGRR SPSDGAREDD EDVDQLFGAY GSSPGPSPGP SPARPPAKPP EDEPDADGYE121SDDCTALGTL DFSLLYDQEN NALHCTITKA KGLKPMDHNG LADPYVKLHL LPGASKANKL181RTKTLRNTLN PTWNETLTYY GITDEDMIRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP241NHTKTFSICL EKQLPVDKTE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG301YSDPYVKTYL RPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKS LEVTVWDYDI361GKSNDFIGGV VLGIHAKGER LKHWFDCLKN KDKRIERWHT LTSELPGAVL SDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
412 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
