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Human DMTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DMTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DMTF1 antibodies →
$153.00each
Catalog Number: LS-G85954-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DMTF1 (NM_021145) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DMTF | HDMP1 | HDMTF1 | DMTF1
Origin Species
Human
NCBI GenBank
NM_021145
SwissProt
Q9Y222

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSTVEEDSDT VTVETVNSVT LTQDTEGNLI LHCPQNEADE IDSEDSIEPP HKRLCLSSED
61DQSIDDSTPC ISVVALPLSE NDQSFEVTMT ATTEVADDEV TEGTVTQIQI LQNEQLDEIS
121PLGNEEVSAV SQAWFTTKED KDSLTNKGHK WKQGMWSKEE IDILMNNIER YLKARGIKDA
181TEIIFEMSKD ERKDFYRTIA WGLNRPLFAV YRRVLRMYDD RNHVGKYTPE EIEKLKELRI
241KHGNDWATIG AALGRSASSV KDRCRLMKDT CNTGKWTEEE EKRLAEVVHE LTSTEPGDIV
301TQGVSWAAVA ERVGTRSEKQ CRSKWLNYLN WKQSGGTEWT KEDEINLILR IAELDVADEN
361DINWDLLAEG WSSVRSPQWL RSKWWTIKRQ IANHKDVSFP VLIKGLKQLH ENQKNNPTLL
421ENKSGSGVPN SNTNSSVQHV QIRVARLEDN TAISSSPMAA LQIPVQITHV SSADSPATVD
481SETITLNSGT LQTFEILPSF HLQPTGTPGT YLLQTSSSQG LPLTLTASPT VTLTAAAPAS
541PEQIIVHALS PEHLLNTSDN VTVQCHTPRV IIQTVATEDI TSSISQAELT VDSDIQSSDF
601PEPPDALEAD TFPDEVHHPK MTVEPSFNDA HVSKFSDQNS TELMNSVMVR TEEEISDTDL
661KQEESPSDLA SAYVTEGLES PTIEEQVDQT IDDETILIVP SPHGFIQASD VIDTESVLPL
721TTLTDPILQH HQEESNIIGS SLGSPVSEDS KDVEDLVNCH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

760 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.