DHX35 Antibody
Catalog Number: LS-C665081-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DHX35 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G86411-20
Catalog Number: LS-G86411
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DHX35 (NM_001190809) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C20orf15 | DEAH box protein 35 | DEAH-box protein 35 | KAIA0875 | DDX35 | DHX35
- Origin Species
- Human
- NCBI GenBank
- NM_021931
- SwissProt
- Q9H5Z1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ RQKLPVFKYL61AEAGWTAEGR VVGVTQPRRV AAVTVAGRVA EERGAVLGHE VGYCIRFDDC TDQLATRIKF121LTDGMLVREM MVDPLLTKYS VIMLDEAHER TLYTDIAIGL LKKIQKKRGD LRLIVASATL181DADKFRDFFN QNETSDPARD TCVILTVEGR TFPVDIFYLQ SPVPDYIKST VETVVKIHQT241EGDGDVLAFL TGQEEVETVV SMLIEQARAL ARTGMKRHLR VLPMYAGLPS FEQMKVFERV301SRSVRKVIVA TNVAETSITI SGIVYVIDCG FVKLRAYNPR TAIECLVVVP VSQASANQRA361GRGGRSRSGK CYRLYTEEAF DKLPQSTVPE MQRSNLAPVI LQLKALGIDN VLRFHFMSPP421PAQSMVQALE LLYALGGLDK DCRLTEPLGM RIAEFPLNPM FAKMLLESGN FGCSQEILSI481AAMMQIQNIF VVPPNQKSHA IRVHRKFAVE EGDHLTMLNI YEAFIKHNKD SKWCQEHFLN541YKGLVRAATV REQLKKLLVK FQVPRKSSEG DPDLVLRCIV SGFFANAARF HSTGAYRTIR601DDHELHIHPA SVLYAEKPPR WVIYNEVIQT SKYYMRDVTA IESAWLLELA PHFYQQGTHL661SLKAKRAKVQ DPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
672 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
