DET1 Antibody
Catalog Number: LS-C682171
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DET1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G86973-20
Catalog Number: LS-G86973
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DET1 (NM_017996) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DET1 | DET1 homolog | De-etiolated 1 | De-etiolated-1 homolog
- Origin Species
- Human
- NCBI GenBank
- NM_017996
- SwissProt
- Q7L5Y6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVGQILKRDV IMDHHVSTIK PRRIQNQNVI HRLERRRISS GKAGTHWHQV RVFHQNVFPN61FTVVNVEKPP CFLRKFSPDG RYFIAFSSDQ TSLEIYEYQG CQAAEDLLQG YEGEILSNGN121DQRSVNIRGR LFERFFVLLH ITNVAANGEH LNRECSLFTD DCRCVIVGSA AYLPDEPHPP181FFEVYRNSES VTPNPRSPLE DYSLHIIDLH TGRLCDTRTF KCDKVVLSHN QGLYLYKNIL241AILSVQQQTI HVFQVTPEGT FIDVRTIGRF CYEDDLLTVS AVFPEVQRDS QTGMANPFRD301PFINSLKHRL LVYLWRRAEQ DGSAMAKRRF FQYFDQLRQL RMWKMQLLDE NHLFIKYTSE361DVVTLRVTDP SQASFFVVYN MVTTEVIAVF ENTSDELLEL FENFCDLFRN ATLHSEVQFP421CSASSNNFAR QIQRRFKDTI INAKYGGHTE AVRRLLGQLP ISAQSYSGSP YLDLSLFSYD481DKWVSVMERP KTCGDHPIRF YARDSGLLKF EIQAGLLGRP INHTVRRLVA FTFHPFEPFA541ISVQRTNAEY VVNFHMRHCC TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
561 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
