Human DEPDC4 Protein (Recombinant His, N-Terminal)
Catalog Number: LS-G144957-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DEPDC4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93819-20
Catalog Number: LS-G93819
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DEPDC4 (NM_152317) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DEP.4 | DEP domain containing 4 | DEPDC4
- Origin Species
- Human
- SwissProt
- Q8N2C3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVPGEEPARE LMAVLLTPRF RRLVSQNELP GPGLNGPSSR NRRDGFCRKR RTGCSGPFQA61TQLWDGIIHS LQAQVEIKRR RHHLQTYKDC FTGSDAVDVV LSHLMQNTCL SSNDISCLKG121VHLCQVLMNH KVFEPVGMKK LFKKEKELEF EDSNISLYRF LGNKSSYDCC KRQKDAENEF181NETLRPGYEM ISNPLAQEIG EERIEELIHT INGNPALCPN ITVQKPFLRL SKEDVWKEQT241LLCLLQLIHL PFLDNILEPP VKTQNLQLNK EEDLVITNTC LDRELIPSLC LPEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
294 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
