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Human DENND2D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DENND2D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DENND2D antibodies →
$153.00each
Catalog Number: LS-G86942-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND2D (NM_024901) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DENN/MADD domain containing 2D | DENND2D | RP5-1180E21.2
Origin Species
Human
NCBI GenBank
NM_024901
SwissProt
Q9H6A0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MEGQVVGRVF RLFQRRLLQL RAGPPQDNSG EALKEPERAQ EHSLPNFAGG QHFFEYLLVV
61SLKKKRSEDD YEPIITYQFP KRENLLRGQQ EEEERLLKAI PLFCFPDGNE WASLTEYPRE
121TFSFVLTNVD GSRKIGYCRR LLPAGPGPRL PKVYCIISCI GCFGLFSKIL DEVEKRHQIS
181MAVIYPFMQG LREAAFPAPG KTVTLKSFIP DSGTEFISLT RPLDSHLEHV DFSSLLHCLS
241FEQILQIFAS AVLERKIIFL AEGLSTLSQC IHAAAALLYP FSWAHTYIPV VPESLLATVC
301CPTPFMVGVQ MRFQQEVMDS PMEEVLLVNL CEGTFLMSVG DEKDILPPKL QDDILDSLGQ
361GINELKTAEQ INEHVSGPFV QFFVKIVGHY ASYIKREANG QGHFQERSFC KALTSKTNRR
421FVKKFVKTQL FSLFIQEAEK SKNPPAGYFQ QKILEYEEQK KQKKPREKTV K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

471 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.