DENND1B Antibody
Catalog Number: LS-C816241-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DENND1B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87216-20
Catalog Number: LS-G87216
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND1B (NM_144977) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1ORF18 | C1orf218 | Connecdenn 2 | DENN/MADD domain containing 1B | Protein FAM31B | DENND1B | FAM31B | RP11-53I24.2
- Origin Species
- Human
- NCBI GenBank
- NM_001142795
- SwissProt
- Q6P3S1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAPREEKR WPQPVFSNPV VLWKFPEDFG DQEILQSVPK FCFPFDVERV SQNQVGQHFT61FVLTDIESKQ RFGFCRLTSG GTICLCILSY LPWFEVYYKL LNTLADYLAK ELENDLNETL121RSLYNHPVPK ANTPVNLSVH SYFIAPDVTG LPTIPESRNL TEYFVAVDVN NMLQLYASML181HERRIVIISS KLSTLTACIH GSAALLYPMY WQHIYIPVLP PHLLDYCCAP MPYLIGIHSS241LIERVKNKSL EDVVMLNVDT NTLESPFSDL NNLPSDVVSA LKNKLKKQST ATGDGVARAF301LRAQAALFGY YRDALRYKPG EPITFCEESF VKHRSSVMKQ FLETAINLQL FKQFIDGRLA361KLNAGRGFSD VFEEEITSGG FCGGKDKLQY DYPFSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
396 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
