DDX4 / VASA Antibody
Catalog Number: LS-C416900
2 sizes / from $312.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DDX4 / VASA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91111-20
Catalog Number: LS-G91111
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX4(DDX4) (NM_001166534) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX4 | DEAD box protein 4 | Vasa homolog | VASA
- Origin Species
- Human
- NCBI GenBank
- S75275
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSRNLFLTN SPESSNDCED NPTRNRGFSK RGDNDLDPDE CMQRTGGLFG SRRPVLSGTG61NGDTSQSRSG SGSERDSWKS EAEGGESSDT QGPKVTYIPP PPPEDEDSIF AHYQTGINFD121KYDTILVEVS GHDAPPAILT FEEANLCQTL NNNIAKAGYT KLTPVQKYSI PIILAGRDLM181ACAQTGSGKT AAFLLPILAH MMHDGITASR FKELQEPECI IVAPTRELVN QIYLEARKFS241FGTCVRAVVI YGGTQLGHSI RQIVQGCNIL CATPGRLMDI IGKEKIGLKQ IKYLVLDEAD301RMLDMGFGPE MKKLISCPGM PSKEQRQTLM FSATFPEEIQ RLAAEFLKSN YLFVAVGQVG361GACRDVQQTV LQVGQFSKRE KLVEILRNIG DERTMVFVET KKKADFIATF LCQEKISTTS421IHGDREQRER EQALGDFRFG KCPVLVATSV AARGLDIENV QHVINFDLPS TIDEYVHRIG481RTGRCGNTGR AISFFDLESD NHLAQPLVKV LTDAQQDVPA WLEEIAFSTY IPGFSGSTRG541NVFASVDTRK GKSTLNTAGF SSSQAPNPVD DESWDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
575 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
