LSBio
Human DDX19B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DDX19B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DDX19B antibodies →
$220.00each
Catalog Number: LS-G89622-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX19B (NM_001014451) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DBP5 | DDX19 | DEAD box RNA helicase DEAD5 | DEAD-box protein 5 | DEAD box protein 19B | DEAD-box RNA helicase DEAD5 | Yeast Dbp5 homolog | DDX19B | RNAH | TDBP
Origin Species
Human
NCBI GenBank
NM_007242
SwissProt
Q9UMR2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MATDSWALAV DEQEAAAESL SNLHLKEEKI KPDTNGAVVK TNANAEKTDE EEKEDRAAQS
61LLNKLIRSNL VDNTNQVEVL QRDPNSPLYS VKSFEELRLP QNLIAQSQSG TGKTAAFVLA
121MLSQVEPANK YPQCLCLSPT YELALQTGKV IEQMGKFYPE LKLAYAVRGN KLERGQKISE
181QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL DEADVMIATQ GHQDQSIRIQ RMLPRNCQML
241LFSATFEDSV WKFAQKVVPD PNVIKLKREE ETLDTIKQYY VLCSSRDEKF QALCNLYGAI
301TIAQAMIFCH TRKTASWLAA ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN
361VCARGIDVEQ VSVVINFDLP VDKDGNPDNE TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI
421LNRIQEHFNK KIERLDTDDL DEIEKIAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

448 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.