DDN / Dendrin Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G113360-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DDN / Dendrin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87288-20
Catalog Number: LS-G87288
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Dendrin(DDN) (NM_015086) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDN | Dendrin | KIAA0749
- Origin Species
- Human
- NCBI GenBank
- AB018292
- SwissProt
- O94850
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLDGPLFSEG PDSPRELQDE ESGSCLWVQK SKLLVIEVKT ISCHYSRRAP SRQPMDFQAS61HWARGFQNRT CGPRPGSPQP PPRRPWASRV LQEATNWRAG PLAEVRAREQ EKRKAASQER121EAKETERKRR KAGGARRSPP GRPRPEPRNA PRVAQLAGLP APLRPERLAP VGRAPRPSAQ181PQSDPGSAWA GPWGGRRPGP PSYEAHLLLR GSAGTAPRRR WDRPPPYVAP PSYEGPHRTL241GTKRGPGNSQ VPTSSAPAAT PARTDGGRTK KRLDPRIYRD VLGAWGLRQG QGLLGGSPGC301GAARARPEPG KGVVEKSLGL AAADLNSGSD SHPQAKATGS AGTEIAPAGS ATAAPCAPHP361APRSRHHLKG SREGKEGEQI WFPKCWIPSP KKQPPRHSQT LPRPWAPGGT GWRESLGLGE421GAGPETLEGW KATRRAHTLP RSSQGLSRGE GVFVIDATCV VIRSQYVPTP RTQQVQLLPS481GVTRVVGDSP SQSKPGKEEG EGATVFPSPC QKRLSSSRLL HQPGGGRGGE AEGGRPGDST541LEERTFRILG LPAPEVNLRD APTQPGSPEH QALGPAASGA QGRAEGSEVA VVQRRAGRGW601ARTPGPYAGA LREAVSRIRR HTAPDSDTDE AEELSVHSGS SDGSDTEAPG ASWRNERTLP661EVGNSSPEED GKTAELSDSV GEILDVISQT EEVLFGVRDI RGTQQGNRKR QSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
711 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.