LSBio
Human DCP1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DCP1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DCP1B antibodies →
$153.00each
Catalog Number: LS-G82621-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DCP1B (NM_152640) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Decapping enzyme hDcp1b | DCP1B | HDcp1b | MRNA-decapping enzyme 1B | DCP1
Origin Species
Human
NCBI GenBank
AA447888
SwissProt
Q8IZD4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVAAGGLV GKGRDISLAA LQRHDPYINR IVDVASQVAL YTFGHRANEW EKTDVEGTLF
61VYTRSASPKH GFTIMNRLSM ENRTEPITKD LDFQLQDPFL LYRNARLSIY GIWFYDKEEC
121QRIAELMKNL TQYEQLKAHQ GTGAGISPVI LNSGEGKEVD ILRMLIKAKD EYTKCKTCSE
181PKKITSSSAI YDNPNLIKPI PVKPSENQQQ RIPQPNQTLD PEPQHLSLTA LFGKQDKATC
241QETVEPPQTL HQQQQQQQQQ QQEKLPIRQG VVRSLSYEEP RRHSPPIEKQ LCPAIQKLMV
301RSADLHPLSE LPENRPCENG STHSAGEFFT GPVQPGSPHN IGTSRGVQNA SRTQNLFEKL
361QSTPGAANKC DPSTPAPASS AALNRSRAPT SVTPVAPGKG LAQPPQAYFN GSLPPQTVGH
421QAHGREQSTL PRQTLAISGS QTGSSGVISP QELLKKLQIV QQEQQLHASN RPALAAKFPV
481LAQSSGTGKP LESWINKTPN TEQQTPLFQV ISPQRIPATA APSLLMSPMV FAQPTSVPPK
541ERESGLLPVG GQEPPAAATS LLLPIQSPEP SVITSSPLTK LQLQEALLYL IQNDDNFLNI
601IYEAYLFSMT QAAMKKTM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

618 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.