DAAM1 Antibody
Catalog Number: LS-C802873
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DAAM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G93585-20
Catalog Number: LS-G93585
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DAAM1 (NM_014992) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0666 | DAAM1
- Origin Species
- Human
- SwissProt
- Q9Y4D1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV EELDVMFSEL61VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT SWPEFYIDQL NSMAARKSLL121ALEKEEEEER SKTIESLKTA LRTKPMRFVT RFIDLDGLSC ILNFLKTMDY ETSESRIHTS181LIGCIKALMN NSQGRAHVLA HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV241LQAMLHYQKY ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF301RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF ELVHIDTKSA361TQMFELTRKR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ YWLLLDRIIQ QIVIQNDKGQ421DPDSTPLENF NIKNVVRMLV NENEVKQWKE QAEKMRKEHN ELQQKLEKKE RECDAKTQEK481EEMMQTLNKM KEKLEKETTE HKQVKQQVAD LTAQLHELSR RAVCASIPGG PSPGAPGGPF541PSSVPGSLLP PPPPPPLPGG MLPPPPPPLP PGGPPPPPGP PPLGAIMPPP GAPMGLALKK601KSIPQPTNAL KSFNWSKLPE NKLEGTVWTE IDDTKVFKIL DLEDLERTFS AYQRQQDFFV661NSNSKQKEAD AIDDTLSSKL KVKELSVIDG RRAQNCNILL SRLKLSNDEI KRAILTMDEQ721EDLPKDMLEQ LLKFVPEKSD IDLLEEHKHE LDRMAKADRF LFEMSRINHY QQRLQSLYFK781KKFAERVAEV KPKVEAIRSG SEEVFRSGAL KQLLEVVLAF GNYMNKGQRG NAYGFKISSL841NKIADTKSSI DKNITLLHYL ITIVENKYPS VLNLNEELRD IPQAAKVNMT ELDKEISTLR901SGLKAVETEL EYQKSQPPQP GDKFVSVVSQ FITVASFSFS DVEDLLAEAK DLFTKAVKHF961GEEAGKIQPD EFFGIFDQFL QAVSEAKQEN ENMRKKKEEE ERRARMEAQL KEQRERERKM1021RKAKENSEES GEFDDLVSAL RSGEVFDKDL SKLKRNRKRI TNQMTDSSRE RPITKLNFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1078 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
