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Human D2HGDH Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human D2HGDH Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all D2HGDH antibodies →
$153.00each
Catalog Number: LS-G94702-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human D2HGDH (NM_152783) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D2HGDH | D2HGD
Origin Species
Human
SwissProt
Q8N465

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPRRPLAWP AWLLRGAPGA AGSWGRPVGP LARRGCCSAP GTPEVPLTRE RYPVQRLPFS
61TVSKQDLAAF ERIVPGGVVT DPEALQAPNV DWLRTLRGCS KVLLRPRTSE EVSHILRHCH
121ERNLAVNPQG GNTGMVGGSV PVFDEIILST ARMNRVLSFH SVSGILVCQA GCVLEELSRY
181VEERDFIMPL DLGAKGSCHI GGNVATNAGG LRFLRYGSLH GTVLGLEVVL ADGTVLDCLT
241SLRKDNTGYD LKQLFIGSEG TLGIITTVSI LCPPKPRAVN VAFLGCPGFA EVLQTFSTCK
301GMLGEILSAF EFMDAVCMQL VGRHLHLASP VQESPFYVLI ETSGSNAGHD AEKLGHFLEH
361ALGSGLVTDG TMATDQRKVK MLWALRERIT EALSRDGYVY KYDLSLPVER LYDIVTDLRA
421RLGPHAKHVV GYGHLGDGNL HLNVTAEAFS PSLLAALEPH VYEWTAGQQG SVSAEHGVGF
481RKRDVLGYSK PPGALQLMQQ LKALLDPKGI LNPYKTLPSQ A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

521 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.