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Human CWC22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CWC22 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CWC22 antibodies →
$153.00each
Catalog Number: LS-G94349-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CWC22 (NM_020943) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CWC22 | EIF4GL | KIAA1604 | NCM | Nucampholin homolog | FSAPb
Origin Species
Human
SwissProt
Q9HCG8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR
61SYDSSMESRN RDREKRRERE RDTDRKRSRK SPSPGRRNPE TSVTQSSSAQ DEPATKKKKD
121ELDPLLTRTG GAYIPPAKLR MMQEQITDKN SLAYQRMSWE ALKKSINGLI NKVNISNISI
181IIQELLQENI VRGRGLLSRS VLQAQSASPI FTHVYAALVA IINSKFPQIG ELILKRLILN
241FRKGYRRNDK QLCLTASKFV AHLINQNVAH EVLCLEMLTL LLERPTDDSV EVAIGFLKEC
301GLKLTQVSPR GINAIFERLR NILHESEIDK RVQYMIEVMF AVRKDGFKDH PIILEGLDLV
361EEDDQFTHML PLEDDYNPED VLNVFKMDPN FMENEEKYKA IKKEILDEGD TDSNTDQDAG
421SSEEDEEEEE EEGEEDEEGQ KVTIHDKTEI NLVSFRRTIY LAIQSSLDFE ECAHKLLKME
481FPESQTKELC NMILDCCAQQ RTYEKFFGLL AGRFCMLKKE YMESFEGIFK EQYDTIHRLE
541TNKLRNVAKM FAHLLYTDSL PWSVLECIKL SEETTTSSSR IFVKIFFQEL CEYMGLPKLN
601ARLKDETLQP FFEGLLPRDN PRNTRFAINF FTSIGLGGLT DELREHLKNT PKVIVAQKPD
661VEQNKSSPSS SSSASSSSES DSSDSDSDSS DSSSESSSEE SDSSSISSHS SASANDVRKK
721GHGKTRSKEV DKLIRNQQTN DRKQKERRQE HGHQETRTER ERRSEKHRDQ NSRGSNWRDP
781ITKYTSDKDV PSERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI
841KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQSR ESKKNQDRRR
901EKSPAKQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

908 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.