CT26 / DDX53 Antibody
Catalog Number: LS-C749953
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CT26 / DDX53 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93579-20
Catalog Number: LS-G93579
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX53 (NM_182699) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancer-associated gene protein | CT26 | DEAD box protein 53 | CAGE | Cancer/testis antigen 26 | DDX53 | DEAD box protein CAGE | DEAD-box protein CAGE
- Origin Species
- Human
- SwissProt
- Q86TM3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP LCFKIKNNMV61GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE MKAKAKAAIE TLIRKQESYN121SESSVDNAAS QTPIGRNLGR NDIVGEAEPL SNWDRIRAAV VECEKRKWAD LPPVKKNFYI181ESKATSCMSE MQVINWRKEN FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV241GILKPTPIQS QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML301VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII ATPGRLNDLQ361MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD RQTVMTSATW PDTVRQLALS421YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE KEKRALTQEF VENMSPNDKV IMFVSQKHIA481DDLSSDFNIQ GISAESLHGN SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY541NYDFPRNIDV YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM601AEQYKLNQQK RHRETRSREP GQRRKEFYFL STRTRPLEQKLISEEDLAANDILDYKDDDDKV
631 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
