LSBio
Human CSF3 / G-CSF Protein (Recombinant)

Biological Activity G-CSF, Human stimulates cell proliferation of M-NFS-60 cells. The ED 50 for this effect is typically 20-100pg/mL.

Human CSF3 / G-CSF Protein (Recombinant)

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Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
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$292.00each
Catalog Number: LS-G27060-10

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Overview

Description
Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by chromatographic techniques, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
C17orf33 | G-CSF | GCSF | Pluripoietin | CSF3 | CSF3OS | Lenograstim
Origin Species
Human
NCBI GenBank
NM_000759|NP_000750.1
SwissProt
P09919

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
18.8 kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA
61PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ
121QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

175 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 0.2 EU/µg
Format
Liquid
Formulation
25 mM Tris-HCl, pH 8.0.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 2 weeks or at -20°c for up to 3 months.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.