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Human COG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COG5 antibodies →
$220.00each
Catalog Number: LS-G89489-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COG5 (NM_006348) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDG2I | COG complex subunit 5 | COG5 | GOLTC1 | GTC-90 | Golgi transport complex 1 | GTC90
Origin Species
Human
NCBI GenBank
NM_006348
SwissProt
Q9UP83

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGWVGGRRRD SASPPGRSRS AADDINPAPA NMEGGGGSVA VAGLGARGSG AAAATVRELL
61QDGCYSDFLN EDFDVKTYTS QSIHQAVIAE QLAKLAQGIS QLDRELHLQV VARHEDLLAQ
121ATGIESLEGV LQMMQTRIGA LQGAVDRIKA KIVEPYNKIV ARTAQLARLQ VACDLLRRII
181RILNLSKRLQ GQLQGGSREI TKAAQSLNEL DYLSQGIDLS GIEVIENDLL FIARARLEVE
241NQAKRLLEQG LETQNPTQVG TALQVFYNLG TLKDTITSVV DGYCATLEEN INSALDIKVL
301TQPSQSAVRG GPGRSTMPTP GNTAALRASL WTNMEKLMDH IYAVCGQVQH LQKVLAKKRD
361PVSHICFIEE IVKDGQPEIF YTFWNSVTQA LSSQFHMATN SSMFLKQAFE GEYPKLLRLY
421NDLWKRLQQY SQHIQGNFNA SGTTDLYVDL QHMEDDAQDI FIPKKPDYDP EKALKDSLQP
481YEAAYLSKSL SRLFDPINLV FPPGGRNPPS SDELDGIIKT IASELNVAAV DTNLTLAVSK
541NVAKTIQLYS VKSEQLLSTQ GDASQVIGPL TEGQRRNVAV VNSLYKLHQS VTKVVSSQSS
601FPLAAEQTII SALKAIHALM ENAVQPLLTS VGDAIEAIII TMHQEDFSGS LSSSGKPDVP
661CSLYMKELQG FIARVMSDYF KHFECLDFVF DNTEAIAQRA VELFIRHASL IRPLGEGGKM
721RLAADFAQME LAVGPFCRRV SDLGKSYRML RSFRPLLFQA SEHVASSPAL GDVIPFSIII
781QFLFTRAPAE LKSPFQRAEW SHTRFSQWLD DHPSEKDRLL LIRGALEAYV QSVRSREGKE
841FAPVYPIMVQ LLQKAMSALQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

860 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.