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Human COG4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COG4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COG4 antibodies →
$220.00each
Catalog Number: LS-G84647-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COG4 (NM_015386) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COD1 | Complexed with Dor1p | COG4 | DKFZP586E1519 | CDG2J | COG complex subunit 4
Origin Species
Human
NCBI GenBank
N73180

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTKMADLDS PPKLSGVQQP SEGVGGGRCS EISAELIRSL TELQELEAVY ERLCGEEKVV
61ERELDALLEQ QNTIESKMVT LHRMGPNLQL IEGDAKQLAG MITFTCNLAE NVSSKVRQLD
121LAKNRLYQAI QRADDILDLK FCMDGVQTAL RSEDYEQAAA HTHRYLCLDK SVIELSRQGK
181EGSMIDANLK LLQEAEQRLK AIVAEKFAIA TKEGDLPQVE RFFKIFPLLG LHEEGLRKFS
241EYLCKQVASK AEENLLMVLG TDMSDRRAAV IFADTLTLLF EGIARIVETH QPIVETYYGP
301GRLYTLIKYL QVECDRQVEK VVDKFIKQRD YHQQFRHVQN NLMRNSTTEK IEPRELDPIL
361TEVTLMNARS ELYLRFLKKR ISSDFEVGDS MASEEVKQEH QKCLDKLLNN CLLSCTMQEL
421IGLYVTMEEY FMRETVNKAV ALDTYEKGQL TSSMVDDVFY IVKKCIGRAL SSSSIDCLCA
481MINLATTELE SDFRDVLCNK LRMGFPATTF QDIQRGVTSA VNIMHSSLQQ GKFDTKGIES
541TDEAKMSFLV TLNNVEVCSE NISTLKKTLE SDCTKLFSQG IGGEQAQAKF DSCLSDLAAV
601SNKFRDLLQE GLTELNSTAI KPQVQPWINS FFSVSHNIEE EEFNDYEAND PWVQQFILNL
661EQQMAEFKAS LSPVIYDSLT GLMTSLVAVE LEKVVLKSTF NRLGGLQFDK ELRSLIAYLT
721TVTTWTIRDK FARLSQMATI LNLERVTEIL DYWGPNSGPL TWRLTPAEVR QVLALRIDFR
781SEDIKRLRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

789 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.