LSBio
Human CNTNAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CNTNAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CNTNAP5 antibodies →
$220.00each
Catalog Number: LS-G95822-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CNTNAP5 (NM_130773) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_130773

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
145.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSLPRLTSV LTLLFSGLWH LGLTATNYNC DDPLASLLSP MAFSSSSDLT GTHSPAQLNW
61RVGTGGWSPA DSNAQQWLQM DLGNRVEITA VATQGRYGSS DWVTSYSLMF SDTGRNWKQY
121KQEDSIWTFA GNMNADSVVH HKLLHSVRAR FVRFVPLEWN PSGKIGMRVE VYGCSYKSDV
181ADFDGRSSLL YRFNQKLMST LKDVISLKFK SMQGDGVLFH GEGQRGDHIT LELQKGRLAL
241HLNLGDSKAR LSSSLPSATL GSLLDDQHWH SVLIERVGKQ VNFTVDKHTQ HFRTKGETDA
301LDIDYELSFG GIPVPGKPGT FLKKNFHGCI ENLYYNGVNI IDLAKRRKHQ IYTGNVTFSC
361SEPQIVPITF VNSSGSYLLL PGTPQIDGLS VSFQFRTWNK DGLLLSTELS EGSGTLLLSL
421EGGILRLVIQ KMTERVAEIL TGSNLNDGLW HSVSINARRN RITLTLDDEA APPAPDSTWV
481QIYSGNSYYF GGCPDNLTDS QCLNPIKAFQ GCMRLIFIDN QPKDLISVQQ GSLGNFSDLH
541IDLCSIKDRC LPNYCEHGGS CSQSWTTFYC NCSDTSYTGA TCHNSIYEQS CEVYRHQGNT
601AGFFYIDSDG SGPLGPLQVY CNITEDKIWT SVQHNNTELT RVRGANPEKP YAMALDYGGS
661MEQLEAVIDG SEHCEQEVAY HCRRSRLLNT PDGTPFTWWI GRSNERHPYW GGSPPGVQQC
721ECGLDESCLD IQHFCNCDAD KDEWTNDTGF LSFKDHLPVT QIVITDTDRS NSEAAWRIGP
781LRCYGDRRFW NAVSFYTEAS YLHFPTFHAE FSADISFFFK TTALSGVFLE NLGIKDFIRL
841EISSPSEITF AIDVGNGPVE LVVQSPSLLN DNQWHYVRAE RNLKETSLQV DNLPRSTRET
901SEEGHFRLQL NSQLFVGGTS SRQKGFLGCI RSLHLNGQKM DLEERAKVTS GVRPGCPGHC
961SSYGSICHNG GKCVEKHNGY LCDCTNSPYE GPFCKKEVSA VFEAGTSVTY MFQEPYPVTK
1021NISLSSSAIY TDSAPSKENI ALSFVTTQAP SLLLFINSSS QDFVVVLLCK NGSLQVRYHL
1081NKEETHVFTI DADNFANRRM HHLKINREGR ELTIQMDQQL RLSYNFSPEV EFRVIRSLTL
1141GKVTENLGLD SEVAKANAMG FAGCMSSVQY NHIAPLKAAL RHATVAPVTV HGTLTESSCG
1201FMVDSDVNAV TTVHSSSDPF GKTDEREPLT NAVRSDSAVI GGVIAVVIFI IFCIIGIMTR
1261FLYQHKQSHR TSQMKEKEYP ENLDSSFRNE IDLQNTVSEC KREYFI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1306 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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