LSBio
Human CNTN1 / gp135 / Contactin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CNTN1 / gp135 / Contactin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CNTN1 antibodies →
$220.00each
Catalog Number: LS-G75305-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Contactin 1(CNTN1) (NM_175038) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNTN1 | Contactin | Contactin 1 | Glycoprotein gp135 | gp135 | Neural cell surface protein F3 | Contactin-1
Origin Species
Human
NCBI GenBank
NM_001843
SwissProt
Q12860

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMWLLVSHL VIISITTCLA VSEEDKGFGP IFEEQPINTI YPEESLEGKV SLNCRARASP
61FPVYKWRMNN GDVDLTSDRY SMVGGNLVIN NPDKQKDAGI YYCLASNNYG MVRSTEATLS
121FGYLDPFPPE ERPEVRVKEG KGMVLLCDPP YHFPDDLSYR WLLNEFPVFI TMDKRRFVSQ
181TNGNLYIANV EASDKGNYSC FVSSPSITKS VFSKFIPLIP IPERTTKPYP ADIVVQFKDV
241YALMGQNVTL ECFALGNPVP DIRWRKVLEP MPSTAEISTS GAVLKIFNIQ LEDEGIYECE
301AENIRGKDKH QARIYVQAFP EWVEHINDTE VDIGSDLYWP CVATGKPIPT IRWLKNGYAY
361HKGELRLYDV TFENAGMYQC IAENTYGAIY ANAELKILAL APTFEMNPMK KKILAAKGGR
421VIIECKPKAA PKPKFSWSKG TEWLVNSSRI LIWEDGSLEI NNITRNDGGI YTCFAENNRG
481KANSTGTLVI TDPTRIILAP INADITVGEN ATMQCAASFD PALDLTFVWS FNGYVIDFNK
541ENIHYQRNFM LDSNGELLIR NAQLKHAGRY TCTAQTIVDN SSASADLVVR GPPGPPGGLR
601IEDIRATSVA LTWSRGSDNH SPISKYTIQT KTILSDDWKD AKTDPPIIEG NMEAARAVDL
661IPWMEYEFRV VATNTLGRGE PSIPSNRIKT DGAAPNVAPS DVGGGGGRNR ELTITWAPLS
721REYHYGNNFG YIVAFKPFDG EEWKKVTVTN PDTGRYVHKD ETMSPSTAFQ VKVKAFNNKG
781DGPYSLVAVI NSAQDAPSEA PTEVGVKVLS SSEISVHWEH VLEKIVESYQ IRYWAAHDKE
841EAANRVQVTS QEYSARLENL LPDTQYFIEV GACNSAGCGP PSDMIEAFTK KAPPSQPPRI
901ISSVRSGSRY IITWDHVVAL SNESTVTGYK VLYRPDGQHD GKLYSTHKHS IEVPIPRDGE
961YVVEVRAHSD GGDGVVSQVK ISGAPTLSPS LLGLLLPAFG ILVYLEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1007 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.