Human CHST7 ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F25554-1
1 plate / $745.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CHST7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91168-20
Catalog Number: LS-G91168
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CHST7 (NM_019886) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C6ST2 | CHST7 | GST-5 | Gn6st-4 | GlcNAc6ST-4 | C6ST-2
- Origin Species
- Human
- NCBI GenBank
- NM_019886
- SwissProt
- Q9NS84
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKGRRRRRRE YCKFALLLVL YTLVLLLVPS VLDGGRDGDK GAEHCPGLQR SLGVWSLEAA61AAGEREQGAE ARAAEEGGAN QSPRFPSNLS GAVGEAVSRE KQHIYVHATW RTGSSFLGEL121FNQHPDVFYL YEPMWHLWQA LYPGDAESLQ GALRDMLRSL FRCDFSVLRL YAPPGDPAAR181APDTANLTTA ALFRWRTNKV ICSPPLCPGA PRARAEVGLV EDTACERSCP PVAIRALEAE241CRKYPVVVIK DVRLLDLGVL VPLLRDPGLN LKVVQLFRDP RAVHNSRLKS RQGLLRESIQ301VLRTRQRGDR FHRVLLAHGV GARPGGQSRA LPAAPRADFF LTGALEVICE AWLRDLLFAR361GAPAWLRRRY LRLRYEDLVR QPRAQLRRLL RFSGLRALAA LDAFALNMTR GAAYGADRPF421HLSARDAREA VHAWRERLSR EQVRQVEAAC APAMRLLAYP RSGEEGDAEQ PREGETPLEM481DADGATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
