LSBio
Human CHKA / CK / Choline Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CHKA / CK / Choline Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CHKA antibodies →
$153.00each
Catalog Number: LS-G75176-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Choline kinase alpha(CHKA) (NM_212469) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CHKA | Choline kinase alpha | CK | CKI | CHETK-alpha | Ethanolamine kinase | CHK | Choline kinase | EK
Origin Species
Human
NCBI GenBank
NM_001277
SwissProt
P35790

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP
61PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL
121SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQVGAEAMV LESVMFAILA ERSLGPKLYG
181IFPQGRLEQF IPSRRLDTEE LGLPDISAEI AEKMATFHGM KMPFNKEPKW LFGTMEKYLK
241EVLRIKFTEE SRIKKLHKLL SYNLPLELEN LRSLLESTPS PVVFCHNDCQ EGNILLLEGR
301ENSEKQKLML IDFEYSSYNY RGFDIGNHFC EWMYDYSYEK YPFFRANIRK YPTKKQQLHF
361ISSYLPAFQN DFENLSTEEK SIIKEEMLLE VNRFALASHF LWGQWSIVQA KISSIEFGYM
421DYAQARFDAY FHQKRKLGV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

439 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.