CDC25C Antibody
Catalog Number: LS-C331630
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CDC25C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77178-20
Catalog Number: LS-G77178
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CDC25C (NM_022809) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC25 | CDC25C phosphatase | CDC25C | CDC25Hu3 | Cell division cycle 25C | Phosphotyrosine phosphatase | PPP1R60 | M-phase inducer phosphatase 3 | Mitosis inducer CDC25
- Origin Species
- Human
- NCBI GenBank
- NM_001790
- SwissProt
- P30307
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTELFSSTR EEGSSGSGPS FRSNQRKMLN LLLERDTSFT VCPDVPRTPV GKFLGDSANL61SILSGSPGFF RTSGSAFSWD DNGNLVDSEM KYLGSPITTV PKLDKNPNLG EDQAEEISDE121LMEFSLKDQE AKVSRSGLYR SPSMPENLNR PRLKQVEKFK DNTIPDKVKK KYFSGQGKLR181KGLCLKKTVS LCDITITQML EEDSNQGHLI GDFSKVCALP TVSGKHQDLK YVNPETVAAL241LSGKFQGLIE KFYVIDCRYP YEYLGGHIQG ALNLYSQEEL FNFFLKKPIV PLDTQKRIII301VFHCEFSSER GPRMCRCLRE EDRSLNQYPA LYYPELYILK GGYRDFFPEY MELCEPQSYC361PMHHQDHKTE LLRCRSQSKV QEGERQLREQ IALLVKDMSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
400 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
