LSBio
Human CD40L Protein (Recombinant Human IgG1 Fc, N-terminus)

Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.

Human CD40L Protein (Recombinant Human IgG1 Fc, N-terminus)

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Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
See all CD40LG antibodies →
$372.00each
Catalog Number: LS-G48667-50

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Overview

Description
CD40L Protein LS-G48667 is a Recombinant Human CD40L produced in HEK 293 Cells Met113-Leu261 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD40LG | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Origin Species
Human
NCBI GenBank
NM_000074
SwissProt
P29965

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
Human IgG1 Fc Tag
Molecular Weight
The recombinant human CD40L/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 42.8 kDa. The apparent molecular mass of rh CD40L/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
Protein Type
Recombinant
Amino Acid Sequence
1Met113-Leu 261

13 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

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