LSBio
Human CD3E + CD3G Protein (Recombinant)

Human CD3E + CD3G Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemOther
Format:Lyophilized
See all CD3E + CD3G antibodies →
$1052.00each
Catalog Number: LS-G97096-50

For bulk orders or custom formulations:

Request a Quote

Overview

Description
The recombinant heterodimer of human CD3E/CD3G comprises 683 (355+328) amino acids and has a calculated molecular mass of 77.2 (40.1+37.1) KDa. As a result of glycosylation, the apparent molecular mass of human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions. A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
Human

Protein

Expression System
Other
Molecular Weight
77.2 kDa
Protein Type
Recombinant
Amino Acid Sequence
1Met1-Asp12 6 and Met1 -Ser116

27 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at -70°C.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.