LSBio
Human CD3E + CD3D Protein (Recombinant)

Human CD3E + CD3D Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemOther
Format:Lyophilized
See all CD3E + CD3D antibodies →
$666.00each
Catalog Number: LS-G97095-50

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Overview

Description
The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40) kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions. A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
Human

Protein

Expression System
Other
Molecular Weight
77.5 kDa
Protein Type
Recombinant
Amino Acid Sequence
1Met 1-Ala  105 and Me t 1-Asp 12 6

31 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Lyophilized
Formulation
Lyophilized from sterile PBS, pH 7.4
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at -70°C.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.