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Human CCNF / Cyclin F Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CCNF / Cyclin F Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G76018-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cyclin F(CCNF) (NM_001761) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cyclin F | FBXO1 | F-box only protein 1 | G2/mitotic-specific cyclin-F | CCNF | Cyclin-F | FBX1
Origin Species
Human
NCBI GenBank
NM_001761
SwissProt
P41002

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSGGVVHCR CAKCFCYPTK RRIRRRPRNL TILSLPEDVL FHILKWLSVE DILAVRAVHS
61QLKDLVDNHA SVWACASFQE LWPSPGNLKL FERAAEKGNF EAAVKLGIAY LYNEGLSVSD
121EARAEVNGLK ASRFFSLAER LNVGAAPFIW LFIRPPWSVS GSCCKAVVHE SLRAECQLQR
181THKASILHCL GRVLSLFEDE EKQQQAHDLF EEAAHQGCLT SSYLLWESDR RTDVSDPGRC
241LHSFRKLRDY AAKGCWEAQL SLAKACANAN QLGLEVRASS EIVCQLFQAS QAVSKQQVFS
301VQKGLNDTMR YILIDWLVEV ATMKDFTSLC LHLTVECVDR YLRRRLVPRY RLQLLGIACM
361VICTRFISKE ILTIREAVWL TDNTYKYEDL VRMMGEIVSA LEGKIRVPTV VDYKEVLLTL
421VPVELRTQHL CSFLCELSLL HTSLSAYAPA RLAAAALLLA RLTHGQTQPW TTQLWDLTGF
481SYEDLIPCVL SLHKKCFHDD APKDYRQVSL TAVKQRFEDK RYGEISQEEV LSYSQLCAAL
541GVTQDSPDPP TFLSTGEIHA FLSSPSGRRT KRKRENSLQE DRGSFVTTPT AELSSQEETL
601LGSFLDWSLD CCSGYEGDQE SEGEKEGDVT APSGILDVTV VYLNPEQHCC QESSDEEACP
661EDKGPQDPQA LALDTQIPAT PGPKPLVRTS REPGKDVTTS GYSSVSTASP TSSVDGGLGA
721LPQPTSVLSL DSDSHTQPCH HQARKSCLQC RPPSPPESSV PQQQVKRINL CIHSEEEDMN
781LGLVRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.