CCDC149 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G105347-100
100 µg (1 mg/mL) / $504.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CCDC149 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G96495-20
Catalog Number: LS-G96495
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CCDC149 (NM_001130726) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEEAMNGDR TESDWQGLVS EYLVCKRKLE SKKEALLILS KELDTCQQER DQYKLMANQL61RERHQSLKKK YRELIDGDPS LPPEKRKQAN LAQLLRDSQD RNKHLGEEIK ELQQRLGEVQ121GDNKLLRMTI AKQRLGDEAI GVRHFAAHER EDLVQQLERA KEQIESLEHD LQASVDELQD181VKEERSSYQD KVERLNQELN HILSGHENRI IDVDALCMEN RYLQERLKQL HEEVNLLKSN241IAKYKNALER RKNSKGQGKS SSSALTGVLS AKQVQDLLSE DHGCSLPATP QSISDLKSLA301TALLETIHEK NMVIQHQRQT NKILGNRVAE LEKKLRTLEV SGLWSLPGGK DTILFSDPTL361PSGQRSRSPL LKFVEQPTEN KADPKDGEAQ KQEEDESCAA AEALTAPEDA GRPAVNSPAN421QSRGNQCKLF HPSLPQLPSE EEVNSLGREI IKLTKEQAAA ELEEVRRESP IEGQRSETGP481APPGLAIQGE LPKSHLDSFE ASRPAAKAST PEDGKGIPEG GGMRSTVKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
529 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
