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Human CASC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CASC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CASC1 antibodies →
$220.00each
Catalog Number: LS-G90896-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CASC1 (NM_018272) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CASC1 | LAS1 | PPP1R54
Origin Species
Human
NCBI GenBank
NM_018272
SwissProt
Q6TDU7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPKAKKSGS KKKKVTKAER LKLLQEEEER RLKEEEEARL KYEKEEMERL EIQRIEKEKW
61HRLEAKDLER RNEELEELYL LERCFPEAEK LKQETKLLSQ WKHYIQCDGS PDPSVAQEMN
121TFISLWKEKT NETFEEVIEK SKVVLNLIEK LKFILLETPP CDLQDKNIIQ YQESILQLQE
181LLHLKFGVAT EILLKQASTL ADLDSGNMEK VIKDENVTLY VWANLKKNPR HRSVRFSETQ
241IGFEIPRILA TSDIAVRLLH THYDHVSALH PVSTPSKEYT SAVTELVKDD VKNVEKAISK
301EVEEESKQQE RGSHLIQEEE IKVEEEQGDI EVKMSSAEEE SEAIKCEREM KVLSETVSAA
361QLLLVENSSE KPDFFEDNVV DLCQFTTLGG VYHLDILELP PQCKPVKGWM IVEILKEGLQ
421KYTYPPETTE EFETENAFPP IEVTLEVHEN VIFFEDPVVV RWDAEGKHWR TDGISNVSYK
481PKERLVTFSL DTFGPVTLIQ DAHINMPYQS WELRPLDVNK VLLTVTTVFT EIQIQIKENL
541CMLSSIKLKD KKHISILEGT WMTPIPFIIA LKEAGLNIFP TRHSHFYVII NNKVPLVEVK
601AYRQMALLSS AFAFGWSKWN LLCNSTKVVF KVREHLTEEC TENPNWALLM FSGDRAQRLK
661IKEESEAFSE ALKEETEFHS TLYHMVKDFA SEEAMEKVRS SNCQFVNSVC HMLLSTRLLS
721YS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

722 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.